| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1166474 | Analytica Chimica Acta | 2012 | 7 Pages |
Abstract
⺠In this study we found that Lys Tag derivatization of lysine-containing peptides leads to side reactions such as methylation, deamidation and Lys Tag labeling of the N-terminus. ⺠We found temperature and pH to be the main variables to control side-reactions. ⺠We show that side-reactions during Lys Tag derivatization can be avoided or minimized by simply lowering the reaction temperature from 55 °C to room temperature.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Olav Mjaavatten, Gyrid Nygaard, Frode S. Berven, Frode Selheim,