| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1166729 | Analytica Chimica Acta | 2011 | 5 Pages |
A two-dimensional (2-D) “heart-cutting” HPLC system was used to fractionate oligostyrenes into the respective diastereoisomers. For samples of known composition, the response of an ultraviolet (UV) absorbance detector followed the anticipated pattern. The response of an evaporative light-scattering (ELSD) detector on the other hand indicated quite different concentrations for the two diastereoisomers, relative to what was anticipated and what was indicated by the UV detector. Whereas approximately the same concentration was indicated by UV, ELSD in some cases indicated no detection of the later eluting isomer. The magnitude of the errors depended on both the molecular weight and the tacticity of the diastereomers. These anomalies appear to be an artifact of power transform functions imbedded within the firmware processor of the ELSD, invisible to the user.
Graphical abstractComparative difference in detection response between UV and ELS detectors in the analysis of oligostyrene diastereomers.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Power transforms might be imbedded in ELS detector firmware. ► Power transforms alter chromatographic peak moments. ► Power transforms can lead to selective detection.
