| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1166776 | Analytica Chimica Acta | 2012 | 5 Pages |
In this paper, a simple and sensitive approach for H5N1 DNA detection was described based on the fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to carbon nanotubes (CNTs) in a QDs-ssDNA/oxCNTs system, in which the QDs (CdTe) modified with ssDNA were used as donors. In the initial stage, with the strong interaction between ssDNA and oxCNTs, QDs fluorescence was effectively quenched. Upon the recognition of the target, the effective competitive bindings of it to QDs-ssDNA occurred, which decreased the interactions between the QDs-ssDNA and oxCNTs, leading to the recovery of the QDs fluorescence. The recovered fluorescence of QDs was linearly proportional to the concentration of the target in the range of 0.01–20 μM with a detection limit of 9.39 nM. Moreover, even a single-base mismatched target with the same concentration of target DNA can only recover a limited low fluorescence of QDs, illustrating the good anti-interference performance of this QDs-ssDNA/oxCNTs system. This FRET platform in the QDs-ssDNA/oxCNTs system was facilitated to the simple, sensitive and quantitative detection of virus nucleic acids and could have a wide range of applications in molecular diagnosis.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The quantum dots-ssDNA probe was designed for the determination of virus DNA. ► The fluorescence of quantum dots was effectively quenched by carbon nanotubes. ► The addition of target H5N1 DNA restored the quenched fluorescence of quantum dots. ► The proposed method exhibited high sensitivity and good selectivity for H5N1 DNA.
