Classification of amino acids and oligopeptides with the use of multi-mode chemical images obtained with ion selective electrode array

The analysis of amino acids and oligopeptides as important bioanalytes is a significant task in medical diagnostics and proteomic research and currently it is based on chromatographic and electrophoretic methods. In this paper, an approach based on sensor array coupled with titration is proposed for effective recognition of 5 amino acids and 4 oligopeptides. It is shown, that the increase of information gained during measurements of sensors’ signals in various pH allows for capturing more data on interaction of amino acids and oligopeptides with various polymeric membranes of potentiometric electrodes used in the array, and that leads to the lowering of classification errors in partial least squares analysis.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► In thos work multi-mode approach for amino acids and oligopeptides analysis was proposed. ► Potentiometric sensor array was used during the titration of aminoacids and oligopeptides. ► The increase of information gained during the titration and multi-mode analysis leads to the lowering of classification errors.