Immunoassay of C-reactive protein by hot electron induced electrochemiluminescence using integrated electrodes with hydrophobic sample confinement

C-reactive protein (CRP) was determined in the concentration range 0.01–10 mg L−1 using hot electron induced electrochemiluminescence (HECL) with devices combining both working and counter electrodes and sample confinement on a single chip. The sample area on the electrodes was defined by a hydrophobic ring, which enabled dispensing the reagents and the analyte directly on the electrode. Immunoassay of CRP by HECL using integrated electrodes is a good candidate for a high-sensitivity point-of-care CRP-test, because the concentration range is suitable, miniaturisation of the measurement system has been demonstrated and the assay method with integrated electrodes is easy to use. High-sensitivity CRP tests can be used to monitor the current state of cardiovascular disease and also to predict future cardiovascular problems in apparently healthy people.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights• C-reactive protein has been determined in the concentration range 0.01–10 mg L−1 using an electrochemiluminescence microchip which employs integrated electrodes with hydrophobic sample confinement. • This arrangement enables very simple and fast CRP analysis amenable to point-of-care applications.