| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1167186 | Analytica Chimica Acta | 2011 | 7 Pages |
Abstract
⺠Two sensitive colorimetric assays were developed to characterize antibody grafting. ⺠These methods are well adapted to pre-screening of antibody grafting performances. ⺠Grafting efficiency and specific activity were measured on two commercial surfaces. ⺠Grafting conditions were optimized in terms of pH buffer and antibody concentration.
Keywords
phosphate buffered saline with Tween 20CBBBCAPBSTHRPDBAEtOHAFMIgGINSmAbPBS3,4-Diaminobenzoic acidBSACoomassie Brilliant Blueopdbovine serum albuminMonoclonal antibodyEthanolortho-phenylenediamineimmunoglobulin GEnzyme-linked immunosorbent assayELISAGrafting efficiencyInitial velocityPhosphate buffered salineSpecific activitydglMethanolMeOHatomic force microscopyAntibodyPAMAMHorseradish peroxidasePolyamidoamine
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Thomas Moreau, Clément Faye, Mickael Baqué, Isabelle Desvignes, Gaëlle Coussot, Robert Pascal, Odile Vandenabeele-Trambouze,