| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1167327 | Analytica Chimica Acta | 2011 | 6 Pages |
Abstract
In this paper, a new chiral stationary phase (CSP) based on Candida antarctica lipase B (CALB) bounding to the surface of macroporous silica gel was developed and its stereoselectivity in enantioseparation and asymmetrical hydrolysis was evaluated. Three CALB-based HPLC columns with different amounts of enzyme immobilized were prepared by employing the immobilization method, namely “in batch”. In this technique two chromatographic supports epoxy silica and aminopropyl silica were considered. This novel CSP was proven capable of hydrolyzing chiral esters asymmetrically as bioreactor and separating several aromatic alcohols and diniconazole enantiomers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zeying He, Chunguang Lv, Xinxin Fan, Zhiqiang Zhou,