Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1167483 | Analytica Chimica Acta | 2010 | 6 Pages |
Abstract
A new rapid method which allows simultaneous one step detection of two analytes of different nature (2,4,6,-trichlorophenol (TCP) and ochratoxin A (OTA)) in red wine was developed. It was based on a column test with three separate immunolayers: two test layers and one control layer. Each layer consisted of sepharose gel with immobilized anti-OTA (OTA test layer), anti-TCP (TCP test layer) or anti-HRP (control layer) antibodies. Analytes bind to the antibodies in the corresponding test layer while sample flows through the column. Then a mixture of OTA-HRP and TCP-HRP in appropriate dilutions was used, followed by the application of chromogenic substrate. Colour development of the test layer occurred when the corresponding analyte was absent in the sample. HRP-conjugates bound to anti-HRP antibody in the control layer independently of presence or absence of analytes and a blue colour developed in the control layer. Cut-off values for both analytes were 2 μg Lâ1. The described method was applied to the simultaneous detection of TCP and OTA in wine samples. To screen the analytes in red wine samples, clean-up columns were used for sample pre-treatment in combination with the test column. Results were confirmed by chromatographic methods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
N.V. Beloglazova, I.Yu. Goryacheva, T.Yu. Rusanova, N.A. Yurasov, R. Galve, M.-P. Marco, S. De Saeger,