Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1167601 | Analytica Chimica Acta | 2011 | 7 Pages |
Abstract
A novel mechanism to detect hydrogen peroxide (H2O2) using a poly(N-butyl benzimidazole) (PBBI)-modified gold (PBBI/Au) electrode is proposed. Synthetic PBBI was oxidized using a mixture of acetic acid (AcOH) and H2O2 to form PBBI N-oxide (PBBINO). The structure of PBBINO was verified by Fourier transform infrared spectroscopy (FT-IR) and the degree of oxidation was measured by X-ray photoelectron spectroscopy (XPS). Moreover, the oxide could be reduced electrochemically back to PBBI. Based on this reaction, a novel enzyme-free PBBI/Au electrode was developed to detect H2O2 in the presence of AcOH electrochemically. The biosensor detected H2O2 linearly over concentrations ranging from 25 μM to 10 mM with a detection limit of 6.25 μM in phosphate buffer solution (PBS) mixed with AcOH at pH 6.4. In addition, at an applied potential of â0.5 V, the sensor characteristics could be tuned using AcOH over a pH range of 3.7-6.4. The sensitivity of the probe could be enhanced from 35.1 to 419.4 μA mMâ1 cmâ2 by modifying the surface morphology of the PBBI/Au electrode from a smooth plane to a granular, three-dimensional configuration. Furthermore, it was not influenced by interfering compounds and showed high thermal stability.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mu-Yi Hua, Hsiao-Chien Chen, Rung-Ywan Tsai, Yu-Chen Lin, Leeyih Wang,