Article ID Journal Published Year Pages File Type
1168229 Analytica Chimica Acta 2009 5 Pages PDF
Abstract

A direct spectrophotometric method for the determination of three artificial colors – amaranth, sunset yellow FCF and tartrazine – in beverages samples is proposed. The spectra were recorded between 359 and 600 nm. The spectra of the samples (just filtrated), pure dyes (concentrations ranged between 0.01 and 1.8 mg L−1 for amaranth, 0.08 and 4.4 mg L−1 for sunset yellow and 0.04 and 1.8 mg L−1 for tartrazine) and synthetic mixtures were disposed in a column-wise augmented data matrix. This kind of data structure, analyzed by multivariate curve resolution-alternating least squares (MCR-ALS) makes it possible to exploit the so called ‘second order advantage’. MCR-ALS algorithm was applied to the experimental data under the non-negativity and equality constraints. As a result, the concentration of each dye in the samples and their corresponding pure spectra were obtained.The results were validated using internal reference materials and no significant differences were found (α = 5%) between the reference values and the ones obtained with the proposed method. The second order advantage made it possible to obtain unbiased results even in the presence of interferences.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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