Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1168254 | Analytica Chimica Acta | 2009 | 6 Pages |
Abstract
The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of Fc-COOH and were easily modified with trialkoxysilane reagents for covalent conjugation of CA 15-3 antibodies (anti-CA 15-3). The Fc-COOH-doped SNPs were characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was demonstrated by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the developed immunosensor showed good linearity at the studied concentration range of 2.0-240Â UÂ mLâ1 with a coefficient 0.9986 and a detection limit of 0.64Â UÂ mLâ1 at S/NÂ =Â 3.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chenglin Hong, Ruo Yuan, Yaqin Chai, Ying Zhuo,