Article ID Journal Published Year Pages File Type
1168946 Analytica Chimica Acta 2008 7 Pages PDF
Abstract

Herein is presented a fully validated method that allows simultaneous determination of nine flavonoid compounds (4 flavan-3-ols and 5 anthocyanins) in skin and seed extracts of red grape varieties. On the other hand, the use of the appropriate standard for each compound studied to build the calibration graphs, avoids the error that implies the quantification of anthocyanins by using the malvidin-3-glucoside as the only reference standard. All these considerations ensure the quantification of the compounds studied in real samples. The high performance liquid chromatography (HPLC) with diode array detector (DAD) method developed exhibits good quality parameters: repeatability with R.S.D. < 5.2% and intermediate precision with R.S.D. < 8.1%.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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