Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1168946 | Analytica Chimica Acta | 2008 | 7 Pages |
Abstract
Herein is presented a fully validated method that allows simultaneous determination of nine flavonoid compounds (4 flavan-3-ols and 5 anthocyanins) in skin and seed extracts of red grape varieties. On the other hand, the use of the appropriate standard for each compound studied to build the calibration graphs, avoids the error that implies the quantification of anthocyanins by using the malvidin-3-glucoside as the only reference standard. All these considerations ensure the quantification of the compounds studied in real samples. The high performance liquid chromatography (HPLC) with diode array detector (DAD) method developed exhibits good quality parameters: repeatability with R.S.D. < 5.2% and intermediate precision with R.S.D. < 8.1%.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Susanna Muñoz, Montserrat Mestres, Olga Busto, Josep Guasch,