Development of an optical surface plasmon resonance biosensor assay for (fluoro)quinolones in egg, fish, and poultry meat

The assay, optimised for detection in three matrices (poultry muscle, fish, and egg), was tested on incurred samples prepared by liquid extraction followed by two washing steps. This rapid, simple method proved adequate for detecting at least 13 (fluoro)quinolones at concentrations below established maximum residue levels (MRLs). The reference molecule norfloxacin could be detected in the range of 0.1-10 μg kg−1 in extracts of egg and poultry meat and in the range of 0.1-100 μg kg−1 in extracts of fish. The determined midpoints of these calibration curves were about 1, 1.5 and 3 μg kg−1 in poultry meat, egg and fish, respectively.