Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1169326 | Analytica Chimica Acta | 2008 | 7 Pages |
Abstract
An electrochemical method to directly detect DNA hybridization was developed on the basis of a new conductive polymer, which was polymerized on the glassy carbon electrode with an indole monomer having a carboxyl group, indole-5-carboxylic acid (ICA). Hybridization with complementary, non-complementary and one-base mismatched DNA targets was studied by cyclic voltammetry (CV). Results showed a significant decrease in the current upon addition of complementary target. The change in peak current that was used as an index of sensor response was found to be linear with target concentration in the range of 3.34 × 10−9 to 1.06 × 10−8 M. The detection limit was 1.0 nM.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xuemei Li, Jianping Xia, Shusheng Zhang,