Article ID Journal Published Year Pages File Type
1170503 Analytica Chimica Acta 2006 5 Pages PDF
Abstract

A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)32+ and alcohol dehydrogenase in sol–gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)32+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol–gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3 × 10−6 M for alcohol (S/N = 3) with a linear range from 2.79 × 10−5 to 5.78 × 10−2 M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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