Article ID Journal Published Year Pages File Type
1170726 Analytica Chimica Acta 2007 8 Pages PDF
Abstract

A rapid and new liquid chromatography–mass spectrometry with ion-trap detection method for the determination of meloxicam (MLX), flunixin meglumine (FLU), carprofen (CPF), and tolfenamic acid (TOLF) in animal tissue is described.MRLs between 10 and 500 μg kg−1 in muscle and between 65 and 1000 μg kg−1 in liver, from different animal species have been established in the EU for these compounds.After chemical hydrolysis, an organic extraction from homogenised tissue was performed. Final extract was injected in a liquid chromatograph with an ion-trap mass spectrometer with electrospray interface.Four identification points (one precursor and two product ions) and a minimum of one ion ratio was monitored for each compound.For quantitative purposes flunixin-D3 (FLU-D3) was used as internal standard.The method was validated using fortified blank muscle and liver from different animal species according to the 2002/657/EC European decision criteria.The decision limits (CCα) and detection capabilities (CCβ) were determined and their values were at concentrations near the MRL for each substance.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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