Investigation on the interaction of tetrachloride fluorescein-bovine serum albumin-β-cyclodextrin and the determination of protein by flow injection analysis

In this paper, a simple and sensitive flow injection analysis (FIA) for the determination of protein with spectroscopic probe was developed. This method was based on the investigation of the interaction of tetrachloride fluorescein (2,4,5,7-tetrachloro-3,6-fluorandiol)-bovine serum albumin (BSA), the coupling reaction of protein with tetrachloride fluorescein (TCFS) which was used as a spectroscopic probe in the presence of β-cyclodextrin (β-CD). The interaction mechanism and the main factors affecting the determination were investigated in details. Under the optimum conditions, the linear range and detection limit were 0.0-28.0 μg mL−1 and 0.76 μg mL−1, respectively. The proposed method has been used to determine albumin in serum albumin with satisfactory results.