| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1171437 | Analytica Chimica Acta | 2006 | 5 Pages |
Abstract
A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33Â ng for AA-I and 7.29Â ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Wan Chan, Ka Man Hui, Wing Tat Poon, Kim Chung Lee, Zongwei Cai,