Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1171720 | Analytica Chimica Acta | 2006 | 5 Pages |
Abstract
A robust, accurate and sensitive automated procedure for the determination of etodolac, an anti-inflammatory drug, in pharmaceutical formulas by sequential injection analysis, is reported. The same system was also applied to evaluate the antioxidant activity of the drug expressed as Trolox equivalent antioxidant capacity (TEAC). The methodology is based on measuring at 734 nm the decay of absorbance of a solution with the radical 2,2â²-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) after its reduction by etodolac. Optimum ABTS+-etodolac reaction was achieved with 0.329 mL of sample and 0.205 mL of ABTS+ solution. Etodolac was determined at concentrations up to 4.5 Ã 10â5 mol Lâ1. A solution detection limit of 6.6 Ã 10â6 mol Lâ1 was obtained under the optimised experimental conditions. A relative standard deviation (n = 10) lower than 4.7% with a sample throughput of more than 21 samples/h was obtained. No interference from excipients was observed. The developed methodology was applied in the analysis of pharmaceutical preparations and the obtained results were in good agreement with those furnished by the reference procedure with relative deviations lower than 1.4%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Julia B. Garcia, M. Lúcia M.F.S. Saraiva, José L.F.C. Lima,