Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1171932 | Analytica Chimica Acta | 2006 | 4 Pages |
Abstract
A microchip capillary electrophoresis system with highly sensitive fluorescence detection is reported. The system was successfully constructed using an inverted fluorescence microscope, a highly sensitive photon counter, a photomultiplier tube (PMT) and a capillary electrophoresis microchip. This system can be applied to the fluorescence detection with various wavelengths (300-600Â nm). Different fluorescence reagents require different excitation wavelengths. The wavelengths of UV light (300-385Â nm), blue light (450-480Â nm) and green light (530-550Â nm) are employed to excite Titan yellow, fluorescence-5-isothiocyanate (FITC) and Rhodamine 6G, respectively. The detection limit (S/NÂ =Â 3) of FITC is 7Â ÃÂ 10â10Â M, which is 2-3 orders of magnitude lower than that obtained with the lamp-based fluorescence and PMT detection system and approaches the data gained by the laser-induced fluorescence detection. The linear relationship is excellent within the range of concentration 1.3Â ÃÂ 10â9 to 6.5Â ÃÂ 10â8Â M FITC. It offers a new method to widen the application of the lamp-based fluorescence detection.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qin Yan, Rong Sheng Chen, Jie-Ke Cheng,