Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1172326 | Analytica Chimica Acta | 2006 | 9 Pages |
Abstract
The application of carbon nanotube polyacrylamide gel electrophoresis (PAGE) is being reported so as to improve selectivity of analysis. A novel polyacrylamide gel was manufactured by adding multi-walled carbon nanotubes (MWNT) when producing the polyacrylamide gel. Carbon nanotube-matrix assistance was used to separate apolipoprotein A-I and complement C3 with native polyacrylamide gel electrophoresis. Significant improvement of the relative selectivity was achieved in the separation between apolipoprotein A-I and complement C3, because of the hydrophobic change of the carbon nanotube surface non-covalently functionalized by the surfactant Triton X-100. These two proteins were identified by MS/MS after electrophoresis. The change of the observed mobility can also be noticed using other nanomaterials such as nano-TiO2 and nano-Al2O3, but no significant improvement of relative selectivities could be observed with the latter nanoparticles.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Guangming Huang, Yangjun Zhang, Jin Ouyang, Willy R.G. Baeyens, Joris R. Delanghe,