| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1172374 | Analytica Chimica Acta | 2006 | 5 Pages |
Abstract
A new substrate, 2-carboxy-1-naphthyl phosphate (CNP), was developed for the fluorimetric determination of alkaline phosphatase (ALP) activity. The product of the enzyme reaction is 1-hydroxy-2-naphthoic acid (HNA), which is a strong fluorescent product. The amount of HNA generated is proportional to ALP activity. Optimal conditions for the determination of ALP were investigated. The linear range and detection limit for the determination of ALP are 0.01-4.8Â U/L and 7.44Â mU/L, respectively. This method is simple, practical and can be successfully applied to assess ALP in human serum with good accuracy and precision. The results were evaluated by comparison with a standard colorimetric assay using p-nitrophenyl phosphate as ALP substrate.
Keywords
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiaojing Zhu, Qikui Liu, Chongqiu Jiang,