Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1172512 | Analytica Chimica Acta | 2006 | 4 Pages |
Even though it has not been widely recognized in the chromatography community, the separation temperature should be considered as an important parameter for the optimization of chromatographic separation conditions. With the help of newly developed stationary phases that are thermally more stable, liquid chromatographic separations may be carried out at elevated temperatures for several thousands of column volumes without decomposition of the packing materials. Therefore, it is important to investigate the temperature effect on column efficiency in liquid chromatographic separations. A mathematical relationship between the plate height and temperature was established in this study. This temperature model is useful for chromatographers to understand the fundamental aspect of the temperature effect in reversed-phase liquid chromatography.