| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1184444 | EuPA Open Proteomics | 2015 | 9 Pages |
•Label-free quantitative proteomics used to study 35 glioma stem cell lines (GSCs).•Discussion of newly reported proteins symplekin and serine/arginine rich splicing factor 2.•Discussion of newly reported upstream regulators IL5 and synoviolin (SYVN1).•Mesenchymal GSCs characterized by a relative decrease in proteins associated with RNA processing.
Glioma stem-like cells (GSCs) are hypothesized to provide a repository of cells in tumors that can self-replicate and are radio- and chemo-resistant. GSC lines, representing several glioma subtypes, have been isolated and characterized at the transcript level. We sought to characterize 35 GSC lines at the protein level using label-free quantitative proteomics. Resulting relative fold changes were used to drive unsupervised hierarchical clustering for the purpose of classifying the cell lines based on proteomic profiles. Bioinformatics analysis identified synoviolin, serine/arginine-rich splicing factor 2, symplekin, and IL-5 as molecules of interest in progression and/or treatment of glioma.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
