Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1267250 | Bioelectrochemistry | 2012 | 7 Pages |
A bio-interphase composed of DNA, cytochrome c (Cyt c) and horseradish peroxidase (HRP) was developed by layer-by-layer assembling Cyt c, DNA and Cyt c-HRP on biocompatible 11-mercaptoundecanoic acid - 6-mercapto-1-hexanol modified gold electrode. The new bio-interphase was used as a model system to mimic the electron transfer and electrocatalytic performance of two proteins in living organisms. Results showed that the electron transfer rate at bi-protein bio-interphase was faster than those at the single protein bio-interphase, indicating a synergistic interaction between the two proteins occurred in the electron transfer. Moreover, the mixed proteins modified electrode exhibited good electrocatalytic response to reduction of hydrogen peroxide (H2O2) and oxygen (O2), suggesting that it could be used as a sensor for H2O2 and O2 detection. The properties of the bio-interphase, together with the bioelectrocatalytic activity, could make it useful in the development of bioelectronic devices, and investigation of electrochemistry of other heme proteins at functional interphase. It would also provide a new strategy for further study on the electron transfer of other multi-proteins in a bio-interphase and the development of biosensors.
Graphical abstractA bio-interphase composed of DNA, cytochrome c (Cyt c) and horseradish peroxidase (HRP) was developed by layer-by-layer assembling Cyt c, DNA and Cyt c-HRP on biocompatible 11-mercaptoundecanoic acid - 6-mercapto-1-hexanol modified gold electrode. The new bio-interphase was used as a model system to mimic electron transfer and electrocatalytic performance of two proteins in living organisms.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A bio-interphase composed of DNA, cytochrome c and horseradish peroxidase was developed. ► The electron transfer between the two proteins was investigated. ► Electrocatalytic performances of the bi-protein modified electrode were explored. ► The bi-protein modified electrode exhibited good electrocatalytic response to O2 and H2O2.