Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1267490 | Bioelectrochemistry | 2006 | 4 Pages |
Abstract
A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant = 103.2 cm− 1) for a real-time conductometric measurement. Tris buffer (2 mM, pH = 7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.
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Authors
Fu-Shiang Chang, Po-Chung Chen, Richie L.C. Chen, Fu-Ming Lu, Tzong-Jih Cheng,