| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1267760 | Bioelectrochemistry | 2016 | 8 Pages |
•Yeast surface displayed CDH communicates with the anode through DET.•Yeast surface displayed CDH possesses a middle point potential of − 28 mV vs. Ag/AgCl.•Yeast surface displayed CDH yielded 3.3 μW cm− 2 in a mediator-less MFC.•Yeast surface displayed PDH yielded 3.9 μW cm− 2 in an air-cathode MFC.
Two dehydrogenases, cellobiose dehydrogenase from Corynascus thermophilus and pyranose dehydrogenase from Agaricus meleagris, were displayed for the first time on the surface of Saccharomyces cerevisiae using the yeast surface display system. Surface displayed dehydrogenases were used in a microbial fuel cell and generated high power outputs. Surface displayed cellobiose dehydrogenase has demonstrated a midpoint potential of − 28 mV (vs. Ag/AgCl) at pH = 6.5 and was used in a mediator-less anode compartment of a microbial fuel cell producing a power output of 3.3 μW cm− 2 using lactose as fuel. Surface-displayed pyranose dehydrogenase was used in a microbial fuel cell and generated high power outputs using different substrates, the highest power output that was achieved was 3.9 μW cm− 2 using d-xylose. These results demonstrate that surface displayed cellobiose dehydrogenase and pyranose dehydrogenase may successfully be used in microbial bioelectrochemical systems.
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