| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1268304 | Bioelectrochemistry | 2011 | 5 Pages |
A glucose biosensor based on a nanocomposite made by layer-by-layer electrodeposition of the redox polymer into a multilayer containing glucose oxidase (GOx) and single-walled carbon nanotubes (SWCNT) on a screen-printed carbon electrode (SPCE) surface was developed. The objectives of the electrodeposition of redox polymer are to stabilize further the multilayer using a coordinative cross-linked redox polymer and to wire the GOx. The electrochemistry of the layer-by-layer assembly of the GOx/SWCNT/redox polymer nanocomposite was followed by cyclic voltammetry. The resultant biosensor provided stable and reproducible electrocatalytic responses to glucose, and the electrocatalytic current for glucose oxidation was enhanced with an increase in the number of layers. The biosensor displayed a linear range from 0.5 to 6.0 mM, a sensitivity of 16.4 μA/(mM cm2), and a response time of about 5 s. It shows no response to 0.05 mM of ascorbic acid, 0.32 mM of uric acid and 0.20 mM of acetaminophen using a Nafion membrane covering the nanocomposite-modified electrode surface.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch Highlights► A carbon nanotube integrated into a multilayer containing glucose oxidase and redox polymer. ► The electron and substrate transfer in the nanocomposition facilitated. ► The electrocatalytic current for glucose oxidation was enhanced with an increase in the number of layers.
