Article ID Journal Published Year Pages File Type
1268934 Bioelectrochemistry 2007 6 Pages PDF
Abstract

A convenient method for the measurement of the catalytic activity of laccase is proposed based on the voltammetric determination of catalytic reaction substrates: 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS) and 1,4-benzenediol (hydroquinone). The measurement performed using microelectrodes working under spherical diffusion conditions is both accurate and simple, and allows to monitor parallely the consumption of substrate and formation of product of the catalytic reaction. The method proposed in this paper was compared with the two generally employed procedures based on oxygen measurement by Clark electrode and on spectrophotometry. The procedure described in the present paper was found to be simpler and more reproducible results were obtained than using Clark electrode. Compared to spectrophotometry a larger range of catalytic reaction substrates can be studied including colorless compounds.

Related Topics
Physical Sciences and Engineering Chemistry Electrochemistry
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