| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1271510 | International Journal of Hydrogen Energy | 2012 | 5 Pages |
Caldicellulosiruptor saccharolyticus is an extreme thermophilic bacterium recognized for its saccharolytic ability and superior ability to produce high yields of hydrogen. However, most studies have been made using yeast extract (YE) as a rich but expensive nutrient source. For the first time, we show that C. saccharolyticus is able to grow on defined minimal media, including essential vitamins, provided that CO2 was allowed to accumulate sufficiently in the culture broth to activate growth. Growth and hydrogen production performance on minimal media was analyzed in both batch and continuous mode. Absence of YE resulted in similar or higher hydrogen yields and specific hydrogen productivities but lower volumetric hydrogen productivities than with YE. The results also indicate that YE is used as a carbon- and energy source thus affecting metabolic flux calculations. This study clarified that YE is not essential making C. saccharolyticus more attractive for fundamental studies on its metabolism and future industrial exploitation.
► Caldicellulosiruptor saccharolyticus was cultured on defined minimal media. ► YE was not required for growth in batch and continuous mode. ► YE had a negative or indifferent effect on H2 yield. ► CO2 was required to activate growth on minimal media. ► Vitamins are required to support rigid growth on minimal media.
