Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1274599 | International Journal of Hydrogen Energy | 2012 | 8 Pages |
Hydrogen is emerging as one of the most promising energy carriers for the future. Most of the methodologies used for hydrogen production employ use of hydrogenases, known to play a critical role in biological hydrogen production as well as its utilization. These hydrogenases display fascinating redox-chemical properties with tremendous efficiency as a biocatalyst for hydrogen fuel. However, Due to the complex structure and maturation process of hydrogenases, their heterologous production has been a challenging task. This results in the limitation of the viable application of this metalloprotein for hydrogen fuel. This review briefly discusses various hydrogenase expression systems and their perspectives.
► Application and limitation of hydrogenases. ► Structure and biosynthesis of hydrogenases. ► Production systems and assay of hydrogenases. ► Protein engineering of hydrogenases.