Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials

In this study, five different flexible osmium based redox polymers were investigated for their ability to efficiently “wire” the oxidoreductase pyranose dehydrogenase (PDH, EC 1.1.99.29) from Agaricus meleagris, on graphite electrodes for possible applications in biofuel cells. A series of newly synthesised osmium based redox polymers covering the potential range between − 270 and + 160 mV vs. Ag|AgCl (0.1 M KCl) was used. The performance of the redox polymers for enzyme wiring was investigated using glucose as substrate. The optimal operational conditions such as pH and potential were investigated.