| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1278753 | International Journal of Hydrogen Energy | 2011 | 6 Pages |
In this work, a novel bio-carrier was used to immobilize photo-fermentative bacteria for hydrogen production. The results showed that the bacterial immobilization and hydrogen production were strongly affected by particle size, amount of bio-carrier and light intensity. Controlling the proper size of bio-carrier not only prevented light shading effect from each other, but also made solid carriers better fluidized during operation. The scanning electronic microscopy revealed that the biofilm formed by photo-fermentative bacteria on the surface of bio-carrier enhanced hydrogen production. Because of bio-carrier fluidization, each immobilized bacterium could receive light energy and produce hydrogen. With the optimal particle size (2 × 2 mm), amount (3% weight to volume ratio) and light intensity (6000 lux), the maximum hydrogen yield of 3.24 mol H2/mol acetate and production rate of 36.06 ml/l/h were obtained in continuous operation stage. Bio-carrier was an effective solid carrier to immobilize photo-fermentative bacteria for improving hydrogen production.
► First time to immobilize photo-fermentative bacteria on fluidized bio-carrier for hydrogen production. ► Each immobilized bacterium could receive light energy and produce hydrogen, due to bio-carrier fluidization. ► Controlling the proper size of bio-carrier not only prevented light shading effect from each other, but also made solid carriers better fluidized during operation. ► With the optimal particle size (2 × 2 mm), amount (3% weight to volume ratio) and light intensity (6000 lux), the maximum hydrogen yield of 3.24 mol H2/mol acetate and production rate of 36.06 ml/l/h were obtained in continuous operation stage.
