Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1281759 | International Journal of Hydrogen Energy | 2008 | 6 Pages |
Abstract
The effects of nutrient limitations on hydrogen yields during fermentation by chemostat cultures of Escherchia coli were studied. Four nutrient regimes (limiting nutrients) were chosen; glucose, nitrogen, sulfur and phosphate. Yields with limiting phosphate or sulfate were low, at or below 1Â mol H2/mol glucose. Yields with continuous cultures limited for nitrogen decreased with increasing dilution rate (D), and were lower than that obtained with a static culture (D=0h-1). The highest yields (at, or somewhat higher than 2Â mol H2/mol glucose) were obtained with cultures limited for glucose. Under most conditions, total hydrogen production and yield were inversely related, with higher production, but lower yields, at increasing dilution rate. A strain, DJT135, mutated in the uptake hydrogenases, in lactate dehydrogenase, and with constitutively active FhlA gave the highest H2 production and greatest H2 to glucose yields.
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Authors
Jonathan Turcot, Ariane Bisaillon, Patrick C. Hallenbeck,