| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1403928 | Journal of Molecular Structure | 2011 | 4 Pages |
In order to obtain unique information of the single gastric cancer cell and investigate gastric cancer further at the cellular and subcellular level, synchrotron infrared (SR-IR) microspectroscopy and imaging was used to study human gastric adenocarcinoma cell line SGC7901 and normal gastric mucosal epithelial cell line GES1. When we compared the infrared micro-spectra of the SGC7901 cells with those of the GES1 cells, the peak height ratios of 1121 cm−1/1020 cm−1 (RNA/DNA) of the SGC7901 cells were significantly higher than those of the GES1 cells, which might be useful for distinguishing malignant cells from normal cells. Furthermore, chemical imaging reveals that the amide II/the amide I ratio of the SGC7901 cell is lower than that of the GES1 cell in the most intense absorption region of vasPO2-. The result indicates the study of the single gastric cancer cell at subcellular level by synchrotron infrared microspectroscopy and imaging can provide more detailed information which is very helpful for the research and diagnosis of gastric cancer.
► Single SGC7901 and GES1 cells were studied by SR-IR microspectroscopy and imaging. ► H1121/H1020 is significantly higher in SGC7901cells than that in GES1 cells. ► Chemical image of the amide II/the amide I of SGC7901is different from that of GES1. ► The differences are useful for distinguishing malignant cells from normal cells.
