| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1405469 | Journal of Molecular Structure | 2014 | 6 Pages |
•Room-temperature X-band EPR spectra of site directed spin labeled hemerythrin, reported for first time.•Viscous media (glycerol, PEG4000, BSA) affect the the mobility (rotational correlation time τc) differently.•Effects on multimeric organization noted.•This study is as part of efforts to design a hemerythrin-based blood substitute.
Reported here are room-temperature continuous wave X-band Electron Paramagnetic Resonance (EPR) spectra of the non-heme di-iron protein hemerythrin (Hr), spin labeled at position 51C in different viscous media, illustrating the mobility and oligomeric recombination tendency of the Phascolopsis gouldii Hr. The mobility of a spin labeled Hr depends on the local viscosity and its connectivity to the nature of the molecular environment (glycerol, PEG4000 and BSA). This provides the basis for a tool useful in directly monitoring Hr in ex vivo samples upon injection within the bloodstream of test animals, for blood substitute research.
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