Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1405492 | Journal of Molecular Structure | 2014 | 6 Pages |
•Ferritin-like iron cores in Azospirillum brasilense Sp245.•Mössbauer spectroscopy with a high velocity resolution.•Heterogeneous iron core structure in bacterial ferritin-like proteins.
The results of a comparative study of two samples of the rhizobacterium Azospirillum brasilense (strain Sp245) prepared in different conditions and of human liver ferritin using Mössbauer spectroscopy with a high velocity resolution demonstrated the presence of ferritin-like iron (i.e. iron similar to that found in ferritin-like proteins) in the bacterium. Mössbauer spectra of these samples were fitted in two ways: as a rough approximation using a one quadrupole doublet fit (the homogeneous iron core model) and using a superposition of quadrupole doublets (the heterogeneous iron core model). Both results demonstrated differences in the Mössbauer parameters for mammalian ferritin and for bacterial ferritin-like iron. Moreover, some differences in the Mössbauer parameters were observed between the two samples of A. brasilense Sp245 related to the differences in their preparation conditions.