Effect of DNA interaction involving antioxidative 4-aminoantipyrine incorporating mixed ligand complexes having alpha-amino acid as co-ligand

•Exploring efficient antioxidative agents having amino acid as co-ligand.•Excellent Kb values for valine mixed ligand complexes.•Effective cleavage of pUC19 DNA via oxidative pathway.•Better antimicrobial active agents.

Few new mixed ligand transition metal complexes of the stoichiometry [ML(A)2], where M = Co(II), Ni(II), Cu(II) and Zn(II), L = FFAP (furfurylidene-4-aminoantipyrine) and A = amino acid (glycine/alanine/valine), have been designed, synthesized and characterized. The molar conductivity of the complexes in DMF at 10−3 M concentration shows that they are non-electrolytes. The interaction of these complexes with CT-DNA indicates that the valine mixed ligand complexes are having higher binding constant than alanine and glycine mixed ligand complexes. This analysis reveals that binding constant depends on the size of the alkyl group present in the amino acid. The binding constants of valine mixed ligand complexes are in the order of 104 to 105 M−1 revealing that the complexes interact with DNA through moderate intercalation mode. The metal complexes exhibit effective cleavage of pUC19 DNA but it is not preceded via radical cleavage and superoxide anion radical. They are good antimicrobial agents than the free ligand. On comparing the IC50 values, [Ni(L)(Gly)2] is considered as a potential drug to eliminate the hydroxyl radical.

Graphical abstractFew novel 4-aminoantipyrine incorporating amino acid mixed ligand complexes act as efficient DNA binding and DNA cleaving agents due to the size of the alkyl group present in the amino acid. They are good antimicrobial and oxidative activators.Figure optionsDownload full-size imageDownload as PowerPoint slide