Article ID Journal Published Year Pages File Type
14110 Biomolecular Engineering 2006 4 Pages PDF
Abstract

The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3′-end mismatches of guessed primers with the target DNA.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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