Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
14110 | Biomolecular Engineering | 2006 | 4 Pages |
Abstract
The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3′-end mismatches of guessed primers with the target DNA.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Fakhraddin Naghibalhossaini, Afsaneh Moaddeb,