Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
14115 | Biomolecular Engineering | 2006 | 14 Pages |
Abstract
Targeted cDNA differential display (TcDD) was developed to study expression of a different selected gene families especially those at low copy numbers per cell. This method is an adaptation of our previously described targeted genomic differential display method (TGDD). In TcDD, the expression of genes containing target sequences such as CAG repeating sequences or genes encoding for zinc-finger binding proteins were followed in an experimental rat model with salt-induced hypertension. DNA sequencing experiments demonstrated that the effectiveness of targeting was greater than 99%.
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Physical Sciences and Engineering
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Authors
Giang H. Nguyen, Nam C. Nguyen, Niels Storm, Chris Gange, Haralambos Gavras, Cassandra L. Smith,