Clinically relevant analytical techniques, organizational concepts for application and future perspectives of point-of-care testing

•This review presents the recent bioanalytical advances for point-of-care testing (POCT).•The underlying techniques concerning microfluidics, nanomaterials, and multiplexing are described.•Various applications of POCT in healthcare are examined concerning clinical relevance.•The organizational concepts for reasonable applications are portrayed.•Another aim of the review is to describe future perspectives of this nascent diagnostic modality.

Applications of near-patient testing have developed rapidly during the last years. It offers quick test results and minimal preanalytical interference, having the potential to improve patient outcomes, even when still under scrutiny by laboratory and healthcare professionals. Near-patient diagnostics are currently also used increasingly in developing countries, due to the burden of inadequate healthcare services in resource-constrained settings.This review describes the underlying emerging techniques that are based on advanced microfluidics and nanomaterials, device miniaturization, and multiplexing the detection mode. The organizational concepts for reasonable applications, contributing significantly to the future perspectives of this nascent diagnostic modality, are supplementary portrayed.