Self-assembled two-dimensional protein arrays in bionanotechnology: from S-layers to designed lattices

•S-layer structure, engineering and technological use are briefly reviewed.•New developments in S-layer protein structure and assembly are presented.•Computational approaches to 2D protein array design are described.•Forthcoming developments are discussed.

Although the crystalline S-layer arrays that form the exoskeleton of many archaea and bacteria have been studied for decades, a long-awaited crystal structure coupled with a growing understanding of the S-layer assembly process are injecting new excitement in the field. The trend is amplified by computational strategies that allow for in silico design of protein building blocks capable of self-assembling into 2D lattices and other prescribed quaternary structures. We review these and other recent developments toward achieving unparalleled control over the geometry, chemistry and function of protein-based 2D objects from the nanoscale to the mesoscale.

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