Synthetic RNA switches as a tool for temporal and spatial control over gene expression

The engineering of biological systems offers significant promise for advances in areas including health and medicine, chemical synthesis, energy production, and environmental sustainability. Realizing this potential requires tools that enable design of sophisticated genetic systems. The functional diversity of RNA makes it an attractive and versatile substrate for programming sensing, information processing, computation, and control functions. Recent advances in the design of synthetic RNA switches capable of detecting and responding to molecular and environmental signals enable dynamic modulation of gene expression through diverse mechanisms, including transcription, splicing, stability, RNA interference, and translation. Furthermore, implementation of these switches in genetic circuits highlights the potential for building synthetic cell systems targeted to applications in environmental remediation and next-generation therapeutics and diagnostics.

Graphical abstract.Figure optionsDownload full-size imageDownload high-quality image (262 K)Download as PowerPoint slideHighlights► RNA is a versatile substrate for encoding sensing, processing, and control functions. ► RNA switches detect molecular and environmental signals to modulate gene expression. ► RNA switches interface with endogenous gene networks to regulate cellular functions. ► Applications include environmental remediation and advanced medical interventions.