| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 16547 | Current Opinion in Biotechnology | 2009 | 7 Pages |
Abstract
Metagenomics has emerged as an alternative approach to conventional microbial screening that allows exhaustive screening of microbial genomes in their natural environments. Despite the potential usefulness of this approach, functional analysis of the metagenome is often problematic because of insufficient and biased expression of the cloned genes in Escherichia coli. This review highlights recent studies on the screening of metagenomic libraries and discusses some possible solutions for overcoming the expression problem in function-based screening.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Taku Uchiyama, Kentaro Miyazaki,