| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 16964 | Enzyme and Microbial Technology | 2015 | 8 Pages |
•We fabricated and modified electrospun polystyrene fibers scaffold for biocatalysis.•We characterized the structures and properties of modified electrospun polystyrene fibers.•We evaluate the enzyme loading efficiency, activity and storage stability of ADH.•Nitration of nanofiber can offer acceptable modification for enzyme immobilization.•Electrospun polystyrene fiber-enzyme assembles can be used for bioconversion of CO2 to biofuel.
Immobilization of the enzymes on nano-structured materials is a promising approach to enhance enzyme stabilization, activation and reusability. This study aimed to develop polystyrene fiber-enzyme assembles to catalyze model formaldehyde to methanol dehydrogenation reaction, which is an essential step for bioconversion of CO2 to a renewable bioenergy. We fabricated and modified electrospun polystyrene fibers, which showed high capability to immobilize dehydrogenase for the fiber-enzyme assembles. Results from evaluation of biochemical activities of the fiber-enzyme assemble showed that nitriation with the nitric/sulfuric acid ratio (v/v, 10:1) and silanization treatment delivered desirable enzyme activity and long-term storage stability, showing great promising toward future large-scale applications.
Graphical abstractPolystyrene nanofiber-enzyme assembles were developed to catalyze model formaldehyde to methanol dehydrogenation reaction, which is an essential step for bioconversion of CO2 to a renewable bioenergy. Electrospun polystyrene fibers modified by low ratio of HNO3/H2SO4 offer high enzyme loading efficiency and reusability. Results showed great an implication of nanostructure-enzyme assembles in future biocatalysts.Figure optionsDownload full-size imageDownload as PowerPoint slide
