| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 17251 | Enzyme and Microbial Technology | 2012 | 5 Pages |
In this report, concentration of malonic acid and acetic acid produced in Escherichia coli were investigated by the expression of acetyl-CoA carboxylase genes (accs) and a malonyl-CoA:ACP transacylase gene (fabD). Both malonyl-CoA and acetyl-CoA are essential intermediate metabolites in the fatty acid biosynthetic pathway, and are reversibly transformed to malonic acid and acetic acid, respectively in the cell. Acetyl-CoA is converted to malonic-CoA by acetyl-CoA carboxylases (Accs), which are composed of 3 different subunits (AccA, AccB, and AccC), and the resulting malonyl-CoA is then converted to malonyl-[acp] by malonyl-CoA:ACP transacylase (FabD). In this study, these genes were separately cloned, and the influences of overexpression of 4 different genes on the concentration of malonic acid and acetic acid were analyzed. Compared with the wild type E. coli, a recombinant strain containing 3 acc genes together showed a 41.03% enhanced malonic acid production, and a 4.29-fold increased ratio of malonic acid to acetic acid.
► Acetyl-CoA carboxylase genes (accs) and a malonyl-CoA:ACP transacylase gene (fabD) were separately cloned. ► The influences of partial overexpression of 4 different genes on the concentrations of malonic acid and acetic acid were analyzed. ► The influence of an expression of acc genes on the production ratio of 2 principal organic acids (e.g., malonic acid and acetic acid) was investigated.
