| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 17254 | Enzyme and Microbial Technology | 2012 | 6 Pages |
Nanobiocatalysis has received growing attention for use in commercial applications. We investigated the efficiency, stability, and reusability of laccase-poly(lactic-co-glycolic acid) (PLGA) nanofiber for diclofenac transformation. NH stretching vibrations (3400–3500 cm−1 and 1560 cm−1) in FT-IR spectra confirmed immobilization of laccase on PLGA nanofibers. The relative activity of immobilized laccase was 82% that of free laccase. Immobilized laccase had better storage, pH, and thermal stability than free laccase. The immobilized laccase produced complete diclofenac transformation in three reuse cycles, which was extended to 6 cycles in the presence of syringaldehyde. Results suggest that laccase-PLGA nanofiber may be useful for removing diclofenac from aqueous sources and has potential for other commercial applications.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The efficiency, stability, and reusability of laccase-PLGA nanofiber for the transformation of diclofenac was investigated. ► A band appearing at NH stretching vibrations in FT-IR spectra confirmed laccase attachment to the PLGA nanofiber. ► Immobilized laccase had better storage, pH, and thermal stability than free laccase. ► Immobilized laccase showed complete diclofenac transformation up to 3 recycles, which could be extended to 6 recycles in the presence of syringaldehyde.
