| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 174811 | Data in Brief | 2016 | 5 Pages |
Abstract
Liver homogenates produced from fasted and non-fasted C57BL/6J female mice were assayed for total lipolytic activity measured as hydrolysis of [9,10-3H(N)]-triolein into [3H] free fatty acids (FFA). Liver homogenates were also used for immunoblotting to determine levels of the lipolytic enzymes adipose-triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), as well as site specific phosphorylation at the 14-3-3 binding site of ATGL and the serine 565 and serine 660 sites of HSL. Significantly higher triolein hydrolysis activity was observed in fasted liver samples, as well as a significant increase in total ATGL and a significant decrease in HSL phosphorylation at the S565 site.
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Physical Sciences and Engineering
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Authors
Phillip M. Marvyn, Emily B. Mardian, Ryan M. Bradley, Kristin A. Marks, Robin E. Duncan,