Article ID Journal Published Year Pages File Type
17690 Enzyme and Microbial Technology 2009 4 Pages PDF
Abstract

To control the molecular mass of a natural polycationic, antimicrobial, Streptomyces-biosynthesized polymer, epsilon-poly-l-lysine, addition of polyanionic cyclodextrin derivatives to the culture medium was evaluated. Chemically modified cyclodextrins such as a sulfated cyclodextrin caused a notable shortening of the polymer length of epsilon-poly-l-lysine from 3.5 to 4.5 kDa to less than 2.5 kDa by the enforcing action of glycerol, which has a weak potential to inhibit polymer elongation by terminal esterification. Meanwhile, polyanionic cyclodextrin inhibited the shortening action with n-octanol, which has a strong ability to inhibit polymer elongation through an undetermined function. The design of chemical structures of polyanionic cyclodextrin, optimization of their addition concentrations, and selection of hydroxyl compounds coexisting with them are critical for this simple and easy method for polymer length control and terminal modification of epsilon-poly-l-lysine.

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