Preparation of bioactive nano-hydroxyapatite coating for artificial cornea

Bioactive coatings containing hydroxyapatite (HA) nano crystals were prepared on PHEMA membrane by a sol dipping method. A cell culture test was used to observe the combination of the material and the cornea fibroblasts. Results show that both the components of the coating and the aggregation degree of HA particles influence the adhesion of the cornea fibroblasts to the membrane, which depends dramatically on the weight ratio between the nano-HA and the dispersant. The optimal condition of the coating preparation is with a dispersant/HA = 1.25 weight ratio. The PHEMA membrane covered with nano-HA coating is promising for artificial cornea use.